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The Art of making Colloidal Silver

Discussion in 'Alt Medicine/Coll Silver' started by abeland1, Mar 16, 2014.



  1. abeland1

    abeland1 Silver Member Silver Miner

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    On post number 174 back in November of last year, I showed a batch made with my new automatic unit measuring 52 ppm. I measured it again before pouring it in the blue bottle. I marked the blue bottle’s label 50ppm before sending it to Mutual Cornell to be analyzed.
    It measured 58 ppm. I don't know why it increased over time while being stored in a clear glass container in normal light. I don't even have a half-baked theory, but this is just one of many samples I have observed that have gained ionic strength in storage.
    IMG_1506 4.jpg
    Here is the report of analysis:
    61969-Atlasnova-retest #3 jpg.jpg

    It measured only 50 ppm. This does not surprise me as I have noted and I think I mentioned somewhere early in this long thread that colloidal silver simply does not travel well. If you think about it the reason is obvious. You have zillions of little tiny charged particles confined in a certain space. If you keep moving them back and forth by vibration enough of them will bounce against each other and against the surface of the container that a certain percentage of them will combine, lose their charge, and no longer be measured or effective. Many years ago I shipped a gallon of colloidal silver from Washington state to Florida and back and noticed a reduction by half of the ionic strength. This is one of the factors that convinced me that colloidal silver needs to be made locally and distributed locally. This fact opens up an opportunity for many.
     

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    Last edited: Aug 1, 2017
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  2. GOLDBRIX

    GOLDBRIX God,Donald Trump,most in GIM2 I Trust. OTHERS-meh Site Supporter Platinum Bling

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    After small family vacation, I came back with enough water bottles to create a half gallon of CS/EIS in the 10 - 12 ppm range.
    I gave my .9999+ fine silver loops a gentle rub with the Scotch Brite pad, filled the half gallon jar with distilled water ( Kroger), flip the switch to the RIGHT for a 10+ppm batch. I got the welcome sign from AtlasNova informing me I was using the CSG-ULTRA, the machine tested the distilled water at 100 and started the electrolysis process by itself.

    I do not sell my production. What I do not use around the house and in washing I do give away to people I think may find it helpful. So no printed labels just a wide strip of masking tape with the information written w/ a Sharpie.

    I'm cookin' up the batch as I type this out.
     
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  3. GOLDBRIX

    GOLDBRIX God,Donald Trump,most in GIM2 I Trust. OTHERS-meh Site Supporter Platinum Bling

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    I just filtered and decanted my half-gallon run of CS/EIS from the AtlasNova CSG-ULTRA ran in the 10ppm mode. The product generated was as clear as the control jar of distilled water. Absolutely no visual difference between the control and the finished production.
    My TDS reading came out at 4 meaning an 8 to 10 est. ppm. ( My TDS meter is set and calibrated for NaCl - Sodium Chloride / Saline solution) and the reason for multiplying by 2 and 2.5 for the estimated silver ppm.
    Tyndall Effect was present in the production and as expected negative in the control sample jar.
    Taste was that of the distilled water base. ( When made with my original generator this level of 10ppm CS/EIS would have a metallic / garden hose like taste)
    For those who have sensitive taste buds ( most of my family) the production of the CSG-ULTRA in the 10ppm +/- range is ideal for oral use and consumption.

    I find it useful to have one machine that can produce such a wide difference of CS/EIS in ppm. Especially with it's Hands Off mode once the production begins.
    In it's first two runs of the AtlasNova CSG-ULTRA I have made a gallon of CS/EIS at at least half the cost of the same quantity of retail purchases in the forms I created, and as far as I'm concerned of equal to better quality. Especially since I know EXACTLY what ingredients are in the production - Good distilled water and .9999+ fine silver, and time ONLY.

    My costs of production of either 50ppm or 10-15ppm can only keep going down with each run I make.
    For all to consider if interested in DIY CS/EIS.

    WAOOR,
    DYODD,
     
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  4. abeland1

    abeland1 Silver Member Silver Miner

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    Scientists find C. diff bacteria hiding in sandboxes:
    http://onlinelibrary.wiley.com/doi/10.1111/zph.12374/full
    I think I just found a great place for my fellow colloidal silver experimenters to discard the batches we don't want or need. Sandboxes are great for small children. It's a place that nurtures their creative instincts. It turns out that it is also a place that has become a breeding ground for newly emerging superbugs, the kind that is not responding to antibiotics. The beauty of it is the fact that the silver particles will mix with and stay in the sand. C. difficile infections can cause diarrhea, other digestive problems, and in some cases, severe colon inflammation. The scientists found genetically different strains of the bacteria, too, including some that were resistant to several antibiotics. The authors say that could pose a real health risk to children exposed to the bacteria while playing. They say their findings should push researchers and local health officials to survey the environment for other potential threats.
     
    Last edited: Aug 1, 2017
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  5. GOLDBRIX

    GOLDBRIX God,Donald Trump,most in GIM2 I Trust. OTHERS-meh Site Supporter Platinum Bling

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    Similar to E. Coli found in ball pits that are not cleaned on a regular basis.

    Good idea Arn. on where to "dump" CS/EIS trials.
     
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  6. abeland1

    abeland1 Silver Member Silver Miner

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    I've been getting some questions and comments on my new automatic colloidal silver generator.
    "Why do I need 50 ppm."
    The main reason is the fact that you want to use silver ions topically or in your bloodstream. Unless you are convinced that you have food poisoning, I don't think that you should drink colloidal silver. Your digestive system depends on a vast array of good bacteria that need to be kept in balance to avoid digestive problems. Some people already have a problem due to our modern diet in this area. Unless you eat something you really shouldn't have, there's no reason to have silver ions in your stomach. Just a sip washed around inside the mouth and absorbed sublingually, or even better daily nose drops are a much better approach. Eye infections are a great target for silver ions. So, as we don't want to swallow ounces of colloidal silver, if we can get the same number of ions in one cc of 50 PPM as we do in 10 ccs of five PPM, we are obviously way ahead. The same thing applies to topical use. Let's say we wipe some 50 ppm on the same area as we would five PPM. There will be ten times as many ions on the same area. Ten times the effect. People who buy this generator will already know that the CS they are already making is effective. The 50 PPM will simply be more effective in all ways by a factor of the ratio of 50 ppm to whatever they are now using. The effectiveness as a deodorant will be enhanced by the same factor. You can use less or use it less often than you do now. Be sure to keep some in the kitchen because you want it handy in case of burns. With a burn, you want the best, and you want it fast.
     
    Last edited: Aug 1, 2017
  7. abeland1

    abeland1 Silver Member Silver Miner

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    http://www.ebay.com/itm/Digital-LCD...610787?hash=item2a86591263:g:bC0AAOSwPh5ZJUiX
    If you're going to have anything to do with colloidal silver, you have to buy one of these. They are now available at any incredibly low price of only $7.86 including shipping. I bought one of these and tested it, and it works just fine. It has enough accuracy to tell you what you must know about your colloidal silver. This is true whether you make your own from plans you see on the Internet or a simple kit or any of the generators that have been mentioned in this thread. If you're making your own, you use this to test for the quality of the distilled water that you're going to use. If it reads anything over two microsiemens, you're not going to be able to make very good colloidal silver. You don't want to have to wait until it turns yellow before you stop your generator. By the time it's yellow, the particles are too large, and the ionic value could go down. If you're using someone's generator, you need one of these to see whether or not it's working properly. You should also have one if you are going to buy colloidal silver by the bottle. If you don't have one of these, how do you know what you have bought? The only part of colloidal silver that is effective is the ionic portion, and that is what this instrument measures. When I first got into colloidal silver and wanted to experiment with different methods of making it, I had to pay $2000 for and an electrical conductivity meter. It was the only thing that was available, and it was a lab quality unit. Say what you want about the Chinese, they have managed to allow us to possess a unit that will do the same thing for under eight dollars including shipping.
    Now if you look at similar items on eBay what is called TDS meters will show up all the way down to $.99. These are not nearly as accurate. A true EC meter reads in microsiemens which have a one-for-one relationship the silver ionic ppm. Many of you may already have the TDS meter and use it by multiplying by two to give an approximate figure for silver ion ppm. This is better than nothing. But for eight dollars, why not get a true reading. This unit also has temperature compensation which is necessary to get an accurate measurement. Some of the cheaper units do not include a battery, so after it arrives, you have to go out and buy a battery. This unit comes with one so you can put it right to work. The only difference between this unit and my $2000 unit is one decimal point.
     
    Last edited: Aug 1, 2017
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  8. GOLDBRIX

    GOLDBRIX God,Donald Trump,most in GIM2 I Trust. OTHERS-meh Site Supporter Platinum Bling

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    I've used a TDS meter for years but if has a One for One reading, and less than 8 bucks w/ FREE SHIPPING.
    Hell, I'll give it a go.

    We Are Our Own Researchers :inspector:
     
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  9. abeland1

    abeland1 Silver Member Silver Miner

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    This has just been posted at silvermedicine@yahoogroups.com :
    "Greetings, Everyone:
    The "experts" are wrong.
    Lenntech states that "under normal conditions, silver is water insoluble". This is the closest statement to the truth while still accidentally misrepresenting the practical aspect of real world use.
    Every chemist that I've talked to quotes wikipedia which states that dissolved silver (as silver oxide...because metalic silver dissolved in water without a salt or protein MUST be an oxide, right?) has a maximum solubility of between 20 - 25 parts per million at room temperature.
    Atlasnova's Ultra automatic EIS generator, which uses a 10 day process with regulated current, produces a stable 50 PPM EIS. The end product is going to turn out to be Over 46 PPM dissolved silver (ions), and under 4 PPM of silver (nano) particles with a negative charge/zeta potential.
    Everyone that I know (and I know a lot of EIS tinkerers) has failed to create a quality product above 24 PPM, including Stephen Quinto, who...at one time... pushed the limits of Argentyn 23 to close to 75 PPM (if I remember correctly) as a part of a failed water purification device experiment. These higher PPM products are simply low quality and unstable.
    The ions simply can't get along, and the particles tend to be very chaotic in the colloid.
    Now, the EIS that I produce is extremely stable. One way to tell is that my meters don't get confused.
    It is a misnomer to say that Arnold's 50 PPM EIS is stable. What I should say, is that it is a highly energized colloid that manages to keep the same amount of silver ions in suspension. The energy of the colloid also either keeps the particles from agglomerating, or the energy keeps breaking them apart perpetually.
    I have a sneaking suspension that it is the latter that is the case.
    Keep in mind that in order to even have a clue about colloidal chemistry, an individual first must have a 4 year chemistry degree. Then, one has to unlearn some of what one learned during that 4 years while pursuing another 4 year degree in colloidal chemistry. Then, there are often sub-specialties for pHds (who is going to do 8 years of chemistry without planning on getting a masters?)
    It is a rare specialty field. I've had the privilege to work with two such individuals due to my work with therapeutic clay. Sadly, both are now retired and don't have access to their fancy (and expensive) toys.
    I even talked to a chemistry professor who taught at Perdue University who claimed that it was not possible to have dissolved silver and a silver colloid together!
    At any rate, at day 24, the conductivity of the sample that I've been studying has actually increased slightly. This active colloid is not going to degrade without interference."

    Silvermedicine.org has been the only noncommercial site for information about colloidal silver for the past 20 years. It is ranked by Google as an "authority site." I sent them one of my new units for them to test and critique and they have done a very thorough job of it. I recommend the site:
    http://www.silvermedicine.org
     
    Last edited: Aug 1, 2017
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  10. abeland1

    abeland1 Silver Member Silver Miner

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    GroupAStrep_CDC-1600x900.jpg

    Last month, a few minor blisters turned into a flesh-eating nightmare for hiker Wayne Atkins, who developed a dangerous bacterial infection after climbing Mount Garfield, a 4,500-foot peak in New Hampshire. He spent 2 1/2 weeks in a medically induced coma while doctors pumped him full of antibiotics and removed chunks of his flesh to get rid of the infection.
    Wayne was lucky, relatively speaking, as flesh-eating bacteria is considered a surgical emergency, and can require limb amputation. One in four people with necrotizing fasciitis dies.
    Around 3 percent of healthy adults and 5 to 15 percent of healthy children have Group A strep bacteria colonies in their nose and throat or on their skin. It becomes flesh eating bacteria when it gets inside a wound. A needle prick or a blister could be enough and it will eventually work its way into the bloodstream. Once these bacteria get underneath the skin they start spreading rapidly, releasing toxins along the way.
    In this post antibiotic age any kind of wound that you have should get your attention. As for myself, I put a drop of 50 PPM EIS on anything that occurs immediately, even a mosquito bite. Or perhaps especially a mosquito bite.
     
    Last edited: Jul 26, 2017
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  11. GOLDBRIX

    GOLDBRIX God,Donald Trump,most in GIM2 I Trust. OTHERS-meh Site Supporter Platinum Bling

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    This should get members to go check up on / read posts in:
    "Colloidal Silver / Electrically Isolated Silver Kills Over 650 Different Microbes
    Discussion in 'Alt Medicine/Coll Silver' started by GOLDBRIX, Dec 17, 2013."

    Just down the list a little way.
     
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  12. abeland1

    abeland1 Silver Member Silver Miner

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    Why you need to equip yourself with the ability to produce the strongest and most effective antimicrobial solution you can.
    This is from the medical news today:
    Has Fleming's warning been ignored?
    We are now at a point where antibiotic resistance has become a serious threat to global public health. In a report earlier this year, Prof. Dame Sally Davies, chief medical officer for England, commented:
    "The soaring number of antibiotic-resistant infections poses such a great threat to society that in 20 years' time we could be taken back to a 19th century environment where everyday infections kill us as a result of routine operations."
    But the threat of antibiotic resistance is not new. As stated previously, Fleming warned of the problem almost 70 years ago. According to Dr. Solomon, such warnings have been overlooked, particularly when the development of antibiotics was at its peak.
    "Although many warnings about resistance were issued, prescribers became somewhat complacent about preserving the effectiveness of antibiotics - new drugs always seemed to be available," he told us. "However, the pipeline for discovery of new antibiotics has diminished in the past 30 years and has now run dry. As bacteria have evolved to resist our current drugs, doctors are now seeing patients with infections that are virtually untreatable."
    He noted, however, that health care providers have now started to become more vigilant in prescribing antibiotics.
    "Greater awareness of the urgency of the problem has given new impetus to careful stewardship of existing antibiotics. Prescribers are now heeding the warning that Fleming gave in his Nobel Prize acceptance speech - to use antibiotics judiciously or else lose them forever."
    Dr. Penn disagrees that warnings of antibiotic resistance have been ignored, telling us that there has been a great deal of research and monitoring of the problem. "The issue has now become much more serious because the supply of new antibiotics is drying up," he added, "and despite the efforts of some, it is clear that antibiotic use globally is still rising fast."
    The barriers halting development of new antibiotics
    Looking back over the past 30 years, there has been astounding progression in the world of medicine. But despite this, there has been a significant decline in research and development of new antibiotics.
    There has been a significant decline in research and development of new antibiotics. Out of 89 new drugs approved by the FDA in 2002, none of them were antibiotics.
    A 2004 report from the Infectious Diseases Society of America (IDSA), for example, found that between 1998 and 2002, approval from the Food and Drug Administration (FDA) for new antibiotics fell by 56%, compared with approval between 1983 and 1987. Furthermore, out of 89 new drugs approved by the FDA in 2002, none of them were antibiotics.
    As a result, we have been relying on the same antibiotics for decades, giving bacteria a better chance to evolve and develop resistance to them. In addition, we have been presented with an array of new infections that are already resistant to currently available antibiotics, such as methicillin-resistant staphylococcus aureus (MRSA).
    The problem is that developing new antibiotics has become a more complex, costly and lengthy process. In a newsletter published by the Alliance for the Prudent Use of Antibiotics (APUA), Dr. Brad Spellburg, assistant professor of medicine at the University of California-Los Angeles (UCLA) and an author of the IDSA report, claims the "low-hanging fruit have been plucked" when it comes to identifying new antibiotics.
    "Drug screens for new antibiotics tend to rediscover the same lead compounds over and over again," he said. "There have been more than 100 antibacterial agents developed for use in humans in the US since sulfonamides. Each new generation that has come to us has raised the bar for what is necessary to discover and develop the next generation."
    He claims that economic factors have interfered with the development of new antibiotics. "The most obvious is that antibiotics are short-course therapies, and companies know that they will make much more money selling a drug you have to take every day for the rest of your life," he said, adding:
    "Also, there are many types of infections, and approval for one type gets a company only one slice of the overall market pie. When antihypertensive drugs are approved, they are not approved to treat hypertension of the lung, or hypertension of the kidney. They are approved to treat hypertension. When antifungals are approved, they are approved to treat 'invasive aspergillosis,' or 'invasive candidiasis.'"
     
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  13. abeland1

    abeland1 Silver Member Silver Miner

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    If you have just come upon this thread and gone ahead to the last post, this one, let me encourage you to start at the very beginning. The purpose of the thread was and remains to encourage and help people to make their own colloidal silver. It has been a learning experience all around. Of late we have been discussing a very advanced procedure and equipment to make a colloidal silver solution, perhaps more properly called EIS or "electrically isolated silver," of much higher potency then has hitherto been achieved. Here we are talking about silver ions, not uncharged silver particles or silver particles coated with some kind of glob, enabling them to stay in solution. Those type of concoctions can go to hundreds of PPM and up and turn you blue. No, if we've done nothing else here, we have proved that is the silver ions that do the work we want. Now this advanced stuff can get to 50 PPM and just as it's a lot better to have a vehicle with 50 HP than with 10 HP, that's very nice. But let's not forget that the 10 HP vehicle will still get you where you want to go. You can make a 10 PPM EIS without buying anything from anybody. You can do it with a couple of Canadian silver Maple leaf coins because they are 9999, some batteries and a simple resistor to limit the amount of current. This will make EIS. It will work. You might just have to use more of it for the same effect. So don't let this thread lead you to the thought that you have to have the 50 ppm level. 10 PPM EIS fix my sinus problem almost 30 years ago, and I would still be snorting that up my nose every morning if I didn't have the 50. With the 50 PPM I now make, I get by just using a few drops in my nose. It's a big deal to me. A slightly bigger deal would be if I had a wound of some kind or something I suspected was really nasty, I would want to use the highest PPM I could. But then again, PPM PPM EIS might be just as effective if you applied it five times as often. So don't think you have to buy a generator from anybody, you don't. Go to the start of the thread, get the fundamentals down pat, and you will be on your way to making your own great health aide.
     
    Last edited: Aug 15, 2017
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  14. louky

    louky Silver Member Silver Miner

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    I'm on the train with an ultra ;)
     
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  15. GOLDBRIX

    GOLDBRIX God,Donald Trump,most in GIM2 I Trust. OTHERS-meh Site Supporter Platinum Bling

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    I like mine. I since you're in KY, and IF you do not make your own distilled water, I have found Kroger's brand of ds works for my machines. Wally World misses more than it hits. With a TDS meter I always get .000 readings and with the ULTRA a reading anywhere from 415 - 425 microamps which the ULTRA likes and will start production.

    I would recommend your first half gallon batch be 10 ppm (Switch RIGHT) in about 7 hours. That way you got a half gallon of effective Electrically Isolated Silver to begin using the first day. That should hold you over while a half gallon batch of 50 ppm processes.

    Anytime you find yourself with some ds water your ULTRA does not like save that ds water for diluting any 50 ppm bottles or jars to levels you wish to use.
    I keep some 50ppm for cuts, burns, and scrapes, and sports gear like Helmets and Shoulder Pads. Some 25 ppm for spraying on my toothpaste, in my liquid bodywash bottle, and add to wash. Some 10 ppm to wipe off food prep areas and use a shot into the family milk jug and with a good shake our milk last far past USE BY date.

    Just for Starters.

    ENJOY
     
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  16. abeland1

    abeland1 Silver Member Silver Miner

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    I just found something out today that surprised me. We have been having a real problem this year with forest fires all around us here in Spokane. We are used to nice clean air here, but we're having a hard time this summer, and for the past two weeks the authorities have advised people not to go out unless they have to, it's that bad. You can feel the effects even indoors. I've been having this itchy little cough caused by irritation at the back of my throat for a few days and cough drops or my regular 10 PPM that I keep in my bathroom for various purposes, did not have any effect. I reopened the jar containing the stuff that I sent to the lab for analysis that I mentioned in one of the posts. It measured 58 when I sent the samples to the lab and arrived there at 50. In any case, I took some and gargled with it. It immediately stopped the irritation that caused a cough. I haven't coughed since. I did it a few more times just to make sure. There was no taste to it, only a slight feeling of numbness. About 3 hours later I felt good enough to have a meal. I found that I had no sense of taste. This level of EIS deadens nerves and taste buds. My sense of taste has now returned. At the 50 ppm level, this EIS appears to be a potent topical anesthetic, something not available without a prescription. That might be of great interest to people with respiratory problems as they would be able to get the same number of silver ions into their lungs using only 1/5 the volume of water they would need with 10 PPM.
     
  17. louky

    louky Silver Member Silver Miner

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    First batch complete. 7 hour, 10ppm or less, clear. Thoughts/questions

    1) happy with the system, makes it easy/comfortable for a newb with auto checks built in
    2) definitely will be getting bleach free coffee filters to strain the "gunk"/film and large visible flecks out
    3) is it normal to turn light yellow hue after 2-3 days (stored in dark cabinet)?
    4) used walgreens distilled water, ultra display screen said passed 87 or 81, something like that. i'm not sure if that's a rating that tells me anything or it it's just a standard pass code.
    5) storage/use bottles? what do you use? definitely need a spray bottle, dropper bottle. store in plastic ever or strictly glass?
    6) no mention of scotch bright cleaning in the instructions, i assume scrub down wire after/before each use?
     
    Last edited: Aug 17, 2017
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  18. abeland1

    abeland1 Silver Member Silver Miner

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    "First batch complete. 7 hour, 10ppm or less, clear. Thoughts/questions"

    "1) happy with the system, makes it easy/comfortable for a newb with auto checks built in"
    I'm glad that you are happy so far, but you can look forward to a lot more with the system. The 10 PPM, 7-hour mode, is part of the system so that people can get going quickly with what is considered good colloidal silver, the same as produced by the quality automatic generators available. Now that you have a good quantity of regular good colloidal silver, you can use the ten-day mode to make the most powerful colloidal silver ever produced, 50 ppm EIS. That is what the ultra is all about.

    "2) definitely will be getting bleach free coffee filters to strain the "gunk"/film and large visible flecks out"
    Yes, the brown ones are the way to go.

    "3) is it normal to turn light yellow hue after 2-3 days (stored in dark cabinet)?
    4) used walgreens distilled water, ultra display screen said passed 87 or 81, something like that. i'm not sure if that's a rating that tells me anything or it it's just a standard pass code."
    If the distilled water that you are using is good, even the 7-hour batches should stay clear. I think the water you used is just on the edge of being rejected. There's nothing wrong with your CS turning slightly yellow. It might be a good idea for you to switch to the Kroger brand as Goldbrix suggested.

    "5) storage/use bottles? what do you use? definitely need a spray bottle, dropper bottle. store in plastic ever or strictly glass?"
    For the 7 hours 10 PPM batches I use the same jugs the distilled water came in. The 50 ppm I store in glass bottles. I have found that 50 ppm is so highly charged that it starts to plate out on plastic.

    "6) no mention of scotch bright cleaning in the instructions, i assume scrub down wire after/before each use?"
    Just a light swipe should be sufficient. You will notice that much less residue is present on the wires on the ten-day mode.

    One thing I've neglected to mention on this thread is that the ten-day mode can be interrupted at any time. The LCD continuously reads out the PPM as it is being generated. So if you run out of CS and you want some right away, you can stop the process at 20, 30 or whatever PPM you want. Of course, once you stopped the process, you won't be able to restart it with the same water, as the system will reject it thinking the distilled water is not good enough.
     
    Last edited: Aug 18, 2017
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  19. GOLDBRIX

    GOLDBRIX God,Donald Trump,most in GIM2 I Trust. OTHERS-meh Site Supporter Platinum Bling

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    abeland1 wrote: "...One thing I've neglected to mention on this thread is that the ten-day mode can be interrupted at any time. The LCD continuously reads out the PPM as it is being generated. So if you run out of CS and you want some right away, you can stop the process it 20, 30 or whatever PPM you want. Of course, once you stopped the process, you won't be able to restart it with the same water, as the system will reject it thinking the distilled water is not good enough".


    I found out the hard way. Dam_ dishwasher puked out water due to a clog in it's drain. Soaked carpeting and linoleum. Insurance sent out a cleaning crew. They put out three industrial size blowers to dry carpet and floor and a dehumidifier for four days. Day three Mrs. decides she needs to run the vacuum in the area where the equipment is running.
    POW goes the circuit breaker and that line just happened to be the one my ULTRA was on.
    Last time I'd looked before the "pop" it had generated 34 ppm on day 7.
    TDS reading indicated 16 ppm when I found the generator had shut itself down. ( TDS meter is calibrated with saline / NaCl so ya multiply by 2 and 2.5 and that gives you a ppm ballpark figure).

    Anyway 30+ppm is good enough for my uses and I still have 50+ ppm in reserve from my first 10 day run.

    Enjoy,
    WAOOR,
     
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  20. louky

    louky Silver Member Silver Miner

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    Good thing I didn't start that 50ppm batch yesterday, my power just went out. Bought the Kroger water. So when power goes out and you're not around, does the ultra flash ppm when it powers back on?
     
  21. abeland1

    abeland1 Silver Member Silver Miner

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    No, the Ultra starts with a clean sheet every time. The microcontroller in the Ultra is what's called a real time computer. If you need to control a process and have response times down to the microseconds you don't have the luxury, as you do on your desktop, of an operating system that allows you to store data and multitask.
    If you have frequent interruptions of power, then you are probably using an uninterruptible supply for your computer if you have a desktop system. You could plug the Ultra into it as the Ultra uses very little power.
    If and when this happens during a ten-day process, no harm is done. After the 1st 24 hours, you have a very stable 10 PPM product so it's not as if it's something that you would want to throw away if the process stopped prematurely. As Goldbrix wrote, when he experienced his power interruption, he still had a half gallon of 34 ppm. That 34 ppm of EIS is still better than anything that you can buy or make with anything else. The so-called gold standard of colloidal silver is supposedly from silver solutions and called our Argentym 23 and sold only to health-care practitioners. That is 11 ppm less than the 34 ppm that Goldbrixis is left with.
    You really should consider buying one of the EC meters on eBay mentioned on previous posts. It will give you a reading within one or 2 ppm of the strength of your EIS. You will find that it will correspond very closely to what your Ultra is telling you what the ppm is at a given time during the process.
     
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  22. GOLDBRIX

    GOLDBRIX God,Donald Trump,most in GIM2 I Trust. OTHERS-meh Site Supporter Platinum Bling

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    Ordered the one you suggested back in July. Just double checked my email notice. It aint overdue until August 23rd . Geesh !!!
    Talk about a slow boat to China. They're slower FROM China.
     
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  23. abeland1

    abeland1 Silver Member Silver Miner

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    Why do you need 50 PPM? If you are talking about using the usual 10 PPM EIS to rinse out your mouth, spray underarms and nether regions, get the gray out, fix our sinuses, cure pinkeye, and heal our burns and wounds, you probably don't. Many thousands of people are getting along just fine using their CSG1s and many other relatively inexpensive colloidal silver generators. Some of these are better than others, but very few are useless. It is one of the great characteristics of EIS that it works even in very light concentrations.
    The new Ultra EIS generator ushers in a new scenario. We are now able to create and store a stable 50 PPM plus EIS by the gallon. This EIS may be viewed as a concentrate enabling us to add it to distilled water at a 5 to 1 rate to generate "normal" EIS. I see the existence of one or 2 gallons of 50 PPM differently.
    The existence of superbugs is not some science fiction fantasy. They exist right now. They are getting stronger every day. It is not only nature that we have to worry about in this regard. There are people who desperately want to harm us, even at the cost of their own well-being, who now have the knowledge, freely available on the net, to engineer the next superbug. Do we know that the EIS, even at 50 PPM, would be effective against such a superbug? No. We do know that, by definition, nothing else is going to work. If you should find yourself or your loved ones the victim of one of the superbugs and the Doctors are throwing up their hands, would you not use all the silver ions you could? There would be no sense in worrying about your gut microbes as they would have been killed off by the antibiotics that the doctors would have already tried. And you wouldn't care about people making silly Smurf jokes, would you?
    The existence of a couple of gallons of stable 50 PPM EIS in your closet should be regarded in the same way as your Glock and your 12gauge.
     
    Last edited: Aug 23, 2017
  24. GOLDBRIX

    GOLDBRIX God,Donald Trump,most in GIM2 I Trust. OTHERS-meh Site Supporter Platinum Bling

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    FYI to ALL Interested : PROPERLY made EIS / CS ( pure distilled water + .9999 Fine or better Ag+ Low electric current ONLY, nothing else used or added) NOBODY in all of history has come down with Argyria aka Blue Skin.
     
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  25. Weatherman

    Weatherman In GIM since 2006 Gold Chaser Site Supporter

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    I am now making my very first CS! I bought the Ultra and set it up with the switch to the right for the first batch of 10 ppm in 7 hours. I will comment later about my results, but here are a few initial observations:

    Water purity: the tap water here has very high TDS and EC. The best bottle water I can use still has some. My new TDS&EC meter reads 75 ppm TDS and 155 us EC. To make the water cleaner, I used the bottle water in a home distiller (similar to post #170), with a count down timer to shut off power while there was still an inch of water left in the distiller. The distilled water reads 1 ppm TDS and 0000 us EC, so it is as clean as I can make it. I used some of that distilled water to rinse out the half gallon jug, and then filled the jug to 1.5 inches from the top.

    Initial Ultra readings: after testing the water quality, the Ultra reported a reading of 91, and began to make the first batch. Soon after starting, the Ultra said the current was 985 uAmps. A few minutes after starting the process, the current had increased to approximately 1050 uAmps, and up to 2025 uAmps after less than a half hour after the start.

    Laser: Quickly after the start, I pointed the red laser beam through the distilled water in the half gallon jug. The beam was weak, but clearly visible in the distilled water. I also pointed the beam through the glass jug that captured the distilled water, but the red beam is not visible in the captured water. That seems strange, but it is what it is.

    Edit to add after approximately 1 hour: The red laser beam is clearly brighter than it was at first. The Ultra reads 2585 uAmps.

    Edit to add after 2 hours: The Ultra reads 3100 uAmps. The red laser beam looks about the same as an hour ago. There are no visible changes in the water, and the water is clear with no trace of a yellow color.

    Edit to add after 3 hours: The Ultra readings alternate between 3150 uAmps, and a few seconds later it reads 1450 uAmps, and then it returns to 3100+ uAmps in the following reading cycle. The red laser beam looks about the same as an hour ago, but I can see tiny traces of brighter flashes in the beam, which could be from larger particles of silver in the water. There are no visible changes in the water, and the water is clear with no trace of a yellow color.

    Edit to add after 5 hours: The Ultra readings alternate between 3200+ uAmps, and a few seconds later it reads 1500+ uAmps, and then it returns to 3200+ uAmps in the following reading cycle. The red laser beam looks about the same as an hour ago, with a few tiny traces of brighter flashes in the beam, which could be from larger particles of silver in the water. There are no visible changes in the water, and the water is clear with no trace of a yellow color. There are three small balls of soft gray "sludge" visible in the bottom of the water jug, and the silver electrodes are showing a small amount of stress with tiny pointed protrusions forming on much of the surface.

    Edit to add after 7 hours: The Ultra used a timer to stop making CS at 7 hours. The red laser beam looks a little brighter than before, with more bright flashes in the beam, which could be from larger particles of silver in the water. There are no visible changes in the water, and the water is clear but with a very small trace of a yellow color. There are the same three small balls of soft gray "sludge" visible in the bottom of the water jug . As I lift the electrodes out of the water, a little gray sludge from the electrodes stays on the water surface. There was a thin light gray layer coating the electrodes, but it was easily removed by gentle rubbing with the included Scotch cleaning pad. My TDS&EC meter reads 24 ppm TDS (increased from 1 at the start) and 11 us EC (increased from 0 at the start). The Ultra did not offer any guess at the CS concentration during the 7 hours it was making CS, but it did "estimate" that the result was 10 PPM of CS.

    I am willing to declare that this first run was a success, so I now have my first CS. I plan to start a 10 day batch of 50 ppm in the next few days.
     
    Last edited: Aug 25, 2017
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  26. Weatherman

    Weatherman In GIM since 2006 Gold Chaser Site Supporter

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    I have started a 10 day run to 50 ppm! Before inserting the silver electrodes into the water, My TDS&EC meter read 0000 TDS and 0000 us, as expected from my distilled water. The Ultra read the initial current at start to be 341 uAmps, and that increased to more than 500 within a few minutes. The Ultra also said that the ppm would be measured later. I will edit below to show the current and ppm over the next few hours, and then each day after.

    Time from start:

    1 hour: 560 uAmps; ppm later
    3 hours: 533 uAmps; Estimated 5 ppm
    6 hours: 553 uAmps; Estimated 7 ppm
    12 hours: 590 uAmps; Estimated 10 ppm
    1 day: 597 uAmps; Estimated 19 ppm
    2 days: 613 uAmps; Estimated 22 ppm
    3 days: 616 uAmps; Estimated 25 ppm
    Water is clear with no color. The electrodes appear to have a light coating. There is no sludge at the bottom of the jar.
    4 days: 615 uAmps; Estimated 29 ppm
    5 days: 618 uAmps; Estimated 32 ppm
    6 days: 619 uAmps; Estimated 36 ppm
    7 days: 618 uAmps; Estimated 40 ppm
    8 days: 619 uAmps; Estimated 43 ppm
    9 days: 617 uAmps; Estimated 47 ppm
    10 days: Auto stop; Estimated 50 ppm
    My meter reads 20 ppm TDS and 40 us EC (compared to 0 and 0 at the start). I am not sure how to translate those readings into the indicated ppm, so I will leave that conversion to others.

    As before, the water is clear with no color:
    1 Clear with no color.jpg

    There is no visible sludge in the bottom, and only a thin coating on the electrodes:
    2 Clear and no sludge.jpg

    After removing the electrodes, the water is clear with only a thin film (dripped off the electrodes) on the surface:
    3 Clear with thin film after remove electrodes.jpg

    The 50 ppm of CS shows a bright laser beam even in bright room light:
    4 Clear and bright laser.jpg

    The removed electrodes showed a dark gray where they were in contact with the water. I used my filter paper to wipe the electrodes first, to save the easily removable silver. Then a quick and easy wipe with the included scrub pad restored the electrodes to looking like new. The only difficulty in this process is taking all the data and photos above.

    The next time I want to make 10 ppm CS, I will use the 50 ppm mode and shut off power after 12 hours. Compared to the 10 ppm mode over 7 hours, the 50 ppm mode runs much less current through the electrodes than the 10 ppm mode, and the CS made with the 50 ppm mode is noticeably clearer and probably better quality. My challenge now is to find ways to use the CS I made, but that will be a good subject for a later post.
     
    Last edited: Sep 6, 2017
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  27. abeland1

    abeland1 Silver Member Silver Miner

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    Thank you, weatherman, for your purchase and even more for your detailed report on the operation of the Ultra Atlasnova colloidal silver generator. Your comment on the 7 hours, 10 PPM mode is well taken. I included this feature in the design of the Ultra so that people could if they desired, make the standard 10 ppm colloidal silver that has long been the standard for colloidal silver generators. It was easy to include it as the algorithm for that mode is trivial when compared to the ten days, 50 ppm mode of operation. You are correct in your comment that if a person wants 10 to 15 ppm, they would be better off to simply stop the 10-day mode after a day. They will have a better 10 PPM product using the more sophisticated algorithm.

    One thing is puzzling about your results. You end up with the reading on your EC metering device of only 40 ppm. This is 20% less than anyone else has ever seen. There are two possibilities. Your EC meter may need to be calibrated. One thing that occurs to me is more likely. In a prior post, you mentioned that the bottled distilled water you bought was not good enough to use. You redistilled it. You may have ended up with a purer distilled water than we have ever encountered. Now the purer the water that is used, the longer the process takes. This is especially true with the principles of the process that I've come up. I have never used twice distilled water. I tried to find some and even bought a gallon through Amazon, found it was rubbish, and returned it. I also bought a distiller that I showed in the previous post and found it faulty, gave up, and threw it out. I decided at that time that I could not sell generators to people that were so critical of water purity that a significant number of them would have to buy a water distiller. Readers of the thread from the beginning will realize that it took me another six months of development to enable the Ultra to work with a wider range of distilled water quality. If you would like to send me your EC meter, I will be happy to calibrate it against my lab instrument and return it to you, but I suspect that the excessive water quality is the problem. Thanks once again for all the useful comments.
     
    Last edited: Sep 6, 2017
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  28. GOLDBRIX

    GOLDBRIX God,Donald Trump,most in GIM2 I Trust. OTHERS-meh Site Supporter Platinum Bling

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    We have entirely different thread for that topic just read down the subject matter list. :2 thumbs up:
     
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  29. chrisflhtc

    chrisflhtc Site Supporter Site Supporter

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    I am really having a hard time understanding why it takes so long for your device to make just 10ppm of colloidal silver. I can make 500ml of 320 ppm in just 3 hours. I run a current of 15ma using a .9999 maple leaf one ounce bullion for the anode and a 12 gauge .9999 wire as the cathode. I use a few drops of sodium carbonate as an electrolyte and several drops of karo syrup to reduce it along with 2 grams of gelatin to cap it so that it is protected from the stomach acids so that it can make it into my blood stream. When it is done it looks like some very strong coffee and one tablespoon diluted in 16 ounces of water makes a perfect pint of 20ppm CS. It works every time and it is very stable with no fallout or degradation at all. I still have some of the first batch I made as a control to compare with subsequent batches. So fare it’s been over a year and it’s still stable. Here are some pics of my process and the dilution. I have a cat who was very sick and in three days giving him 3ml of the 320ppm three times a day he has bounced back like he has no problems, it got rid of his cough and he is eating again the snot he had running out of his nose is all gone. I have also had very good luck knocking a cold I got as well. I started using shots of 320ppm twice a day as well as using a nasal mister twice a day on the first sign of feeling the cold come on and it never got past the feeling of getting sick no congestion no coughing just good health. Now for external use I don’t use the gelatin I just make 500ml of 20ppm and don’t cap it I just reduce it with the karo. Ionic Silver is bad for you it reduces and becomes silver chloride in you stomach. I would never ingest it that’s how you become a smurf as you say we are our own best researchers DYODD

    Calculate the required time to make 20 ppm based on the formula that 1 milligram of silver will enter the water for each 15 milliAmp minutes of process time. 15 milliAmp-minutes could be 1 mA for 15 minutes, 2 mA for 7.5 minutes, 15 mA for 1 minute, etc.


    20 ppm is 20 milligrams of silver per liter. So for example, if you only wanted to make 250ml, you would only need 5 milligrams of silver, and 5 * 15 milliAmp-minutes of current.

    When the required time has elapsed, turn off the power, remove the electrodes, and add 2 drops of the corn syrup solution to the water and heat it to at least 140 degrees F. In a few minutes, it will change from crystal clear and colorless to crystal clear but yellow colored. This color change is the proof that the ionic silver has been converted to true colloidal silver.

    Time required for 1 liter of 20 ppm Colloidal silver at various constant currents:


    Current mA Time minutes
    3 = 100
    5 = 60
    6 = 50
    10 = 30
    15 = 20


    320ppm and 20ppm
    upload_2017-9-7_9-4-47.png
    my stiring hotplate
    upload_2017-9-7_9-5-50.png
    Very clear no turbidity
    upload_2017-9-7_9-7-22.png
     
  30. GOLDBRIX

    GOLDBRIX God,Donald Trump,most in GIM2 I Trust. OTHERS-meh Site Supporter Platinum Bling

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    RIGHT THERE ! You have left Electrically Isolated Silver / Colloidal Silver and have entered the realm of Silver Solutions and Silver Compounds.

    You are sacrificing quality for time, AND adding the potential for Argyria.

    Silver Compounds and Solutions are historically where Argyria issues arrive. EIS/CS = No history of Argyria.

    May I suggest you read up on the late Paul Karason [sp?]. ARGYRIA DID NOT KILL "PAPA Smurf" a heart attack did.
    But he got psychologically addicted to the blue tinge and notoriety and proceeded to make it worse with silver salves, creams and tanning lights. READ UP.

    He is probably the main reference when people claim EIS / CS is "dangerous". SOLUTIONS and COMPOUNDS cause blue tinge but not "dangerous".
     
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  31. chrisflhtc

    chrisflhtc Site Supporter Site Supporter

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    You did say read up I have done extensive reading if you are open minded as you say read this here and let me know what you think https://www.cgcsforum.com/index.php?topic=1141.0 I dare you to read it!
    We are our own best researchers DYODD as you say
    I like reading things you write GOLDBRIX and I love GIM its all good.
    I'm not trying to pee on anyone's cornflakes :)

    solution

    (səˈluːʃən)
    n
    1. (Chemistry) a homogeneous mixture of two or more substances in which the molecules or atoms of the substances are completely dispersed. The constituents can be solids, liquids, or gases
    2. (Chemistry) the act or process of forming a solution
    3. (Chemistry) the state of being dissolved (esp in the phrase in solution)
    4. (Chemistry) a mixture of two or more substances in which one or more components are present as small particles with colloidal dimension; colloid: a colloidal solution.
    ...
    com·pound (kŏm-pound′, kəm-, kŏm′pound′)
    ...

    3. Chemistry A pure, macroscopically homogeneous substance consisting of atoms or ions of two or more different elements in definite proportions that cannot be separated by physical means.
    ...


    Your example; Paul did that to him self. What I am making is nothing like he used to do it to himself.
    I am not sacrificing any quality for time my particle size is very uniform all my batches have the same color and are stable[key word here] .
    We are both making "a solution" and I am making a compound also which is a good thing(see above where it says cannot be separated). Particle agglomeration does not occur when the silver particles are coated with a capping agent so my nano particles don't grow. As a consequence my CS is very stable with no fallout at all. Search capping CS or silver nano particles. Neither of the other elements I use are toxic in the least bit. the gelatin coats the nano particles so that they can make it to the small intestines where it can be absorbed into the blood stream otherwise it will be converted into a chloride in the stomach. I have no horse in this race and am not trying to sell anything but I have read a lot and the place I got the best information is where I copied the following:

    Reducing Agents:

    Reducing agents are necessary if one wishes to make non-ionic colloidal silver. While heat alone can accomplish this, the process is slow, and not always complete. Also, it is impossible to make higher ppm concentrations of silver using heat alone as the silver oxide exceeds its solubility and precipitates out before heat reduction occurs.

    As with electrolytes, the non-toxicity of a reducing agent is the first consideration. I pondered this for a long time until I realized that any food we eat will undergo chemical oxidation by the body. The body knows quite well how to handle the oxidized byproducts of metabolism. This means that if the food is non-toxic, so will be its reduction/oxidation products. Luckily, there are many sugar based and other food products which are reducing agents and work with silver. The quality of the product differs with choice of reducing agents in that some produce more consistent particle sizes, some work faster, and some produce more stable product. Agents which have shown to work are glucose, corn syrup, pure light honey, maltose, maltodextrin, and cinnamon extract. Tea is also a reducing agent, and has been shown to reduce gold. So far, one of the best ones I have found is clear corn syrup. Corn syrup is a 50/50 mixture of glucose and maltose plus water. Both glucose and maltose are reducing agents for silver. Ordinary table sugar (sucrose) does not work, nor does ordinary starch.

    When the process is complete, and all of the silver ions are reduced, the solution will contain nothing toxic.

    For any of these agents to work, the pH of the solution must be basic (above pH 7). pH above 7 opens up the ring structure of a glucose molecule activating it as a reducing agent. Using sodium carbonate as an electrolyte automatically raises the pH sufficiently to activate the reducing agent.

    One ml of 1 Molar sodium carbonate per liter/quart is a very good amount to use, as it is sufficient to prevent plateout, sufficient to activate sugar based reducing agents, and provides plenty of conductivity. Note that different size dropper tips will dispense more or less liquid, so you can calibrate your dropper by counting the number of drops required to fill a teaspoon with your electrolyte and divide that by 5.

    1) A mole of any substance contains approximately 6 x 1023 molecules, and weighs the sum of its atomic weights in grams.
    2) Coffenol developer contains Folgers instant coffee, vitamin C, and baking soda.
    3) Anions are ions which are more negative than the anode. Cations are ions which are more positive than the cathode.
    4) It gives the salt and vinegar flavor to certain brands of potato chips.
    I) Distilled water + silver anode at room temperature.

    In this method, free hydroxyl ions (OH-) in the water initially react with the positive silver electrode to make silver hydroxide (AgOH). Starting with pure water, and pure silver, Silver hydroxide is the only product that can be initially made. Silver hydroxide is unstable though and rapidly decomposes to silver oxide Ag2O. If you remember your high school chemistry, the reaction forumula would be:

    2AgOH –> Ag2O + H2O

    Silver Oxide is slightly soluble in water, and after electrolyzing for a while you have an ionic silver solution, not colloidal silver. You can prove that silver ions exist at this point by adding a small amount of table salt as a test. The salt will form silver chloride which will precipitate out to form a cloudy liquid because the solubility of silver chloride is 25 times less than silver oxide.1

    If the electrolysis is continued, the silver oxide will reach saturation, and then will start to precipitate as colloidal silver oxide. At this point, the solution will start to show the Tyndall effect. This is not strictly colloidal silver, although it does have anti-microbial properties according to the EPA2 Silver oxide is what gives the solution its metallic taste which is another indication you have made silver oxide instead of colloidal silver. (shamelessly lifted from here https://www.cgcsforum.com/index.php?topic=1142.0)


    This is what most people make and erroneously call colloidal silver. Once swallowed and mixed with hydrochloric stomach acid, the silver oxide reacts with the acid producing silver chloride. Once absorbed into the bloodstream, it can travel into the skin and other tissues where it can further react with selenium and sulfer compounds forming silver selenide and silver sulfide. Scanning electron microscopy studies of Argyria victims show that the silver trapped in the skin is predominately silver sulfide and silver selenide, so it is highly likely that ingesting large amounts of ionic silver will eventually lead to Argyria.

     
  32. GOLDBRIX

    GOLDBRIX God,Donald Trump,most in GIM2 I Trust. OTHERS-meh Site Supporter Platinum Bling

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    Been there, read most. Yep, Maybe I'm Old School. "If it aint broke I'm not fixing it", and the EIS I make does what I need it to do. I don't want to wash clothes in corn syrup, or wipe counter tops with it, but hey that's just me.

    On this I agree. The term "Toxic" means poison and even your additives does not make your solution toxic. My fear is you open the door to Argyria with additives.

    NOBODY in recorded history has develop Argyria using ONLY .999+ fine silver electrodes, distilled water, and low voltage. Through stomach acid or sublingually, topical patch, mist inhaler or as a lavage.

    There is evidence where ONLY .999+ fine silver, distilled water, and low voltage HAS remedied mild cases of Argyria.

    We Are Our Own Researchers.
    If you feel comfortable in what you do and how you do it have at it we are all adults here.
     
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  33. abeland1

    abeland1 Silver Member Silver Miner

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    chrisflhtc says:
    chrisflhtc says:
    " I dare you to read it! "
    There is no need to issue a challenge to the readers of the thread. It is safe to say that the followers of this thread will be able to read something without becoming mesmerized. Before we even start to read it we see that it is not a "forum", in the sense that goldismoney2 is a forum. It is the landing page for a company selling colloidal silver generators.
    Under the paragraph titled "scientific studies" he says:
    " There is a published peer reviewed study showing that silver nanoparticles are more effective for bacteria and fungi than ionic silver."Interestingly, AgNP's have been shown in a variety of cases to be more toxic to bacteria and fungi than free ions"7
    It will be left to the reader to seek out the published literature if so desired."
    Why not give us links to these authoritative studies? Here's a link from an unimpeachable source that supports the opposite view:
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2292600/?report=classic
     
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  34. chrisflhtc

    chrisflhtc Site Supporter Site Supporter

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    I work at NIH and have been there for thirty years and have been highly involved in Medical Research the whole time. As I said I have no horse in this race just trying to add a little information to the mix. Here is my email if you wish to email me personally milihrac@mail.nih.gov (this is not my professional opinion) I am not involved in the silver nano particle research personally but have been an avid reader for some time and spent a lot of time talking to those that are. I have nothing to sell unlike yourself. I am just trying to put out some information. People like you piss me off because you have something to sell and post misinformation to do it. You are a snake oil salesman pushing your product! A TEN day process to produce something that should never take that long! The site is there, there is some very good information for someone who wants to find out what they are putting in their bodies. There are a lot of studies listed on the site that I have tried to direct people to. I am not trying to take anything from you hell I bought my first generator from you as I posted earlier in this this thread, I wont try any more. You also sell the lasers that you say are necessary to 'YOUR process along with your TDS meters ( really! read up on what they actually measure it's not silver particles it dissolved solids ? ) . I could care less. I came to GIM looking for information, I found good stuff everywhere but here you seem to be afraid of people learning about what is real. Show me a "formula" that can give repeatable results( ten days to the minute or to the hour?), give me a Break I'm done here on this subject. As I said I'm not trying to piss on anyone's cornflakes but I couldn't stay quite in the face of this misinformation !
     
  35. chrisflhtc

    chrisflhtc Site Supporter Site Supporter

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    "You said"

    Been there, read most. Yep, Maybe I'm Old School. "If it aint broke I'm not fixing it", and the EIS I make does what I need it to do. I don't want to wash clothes in corn syrup, or wipe counter tops with it, but hey that's just me.



    On this I agree. The term "Toxic" means poison and even your additives does not make your solution toxic. My fear is you open the door to Argyria with additives.

    NOBODY in recorded history has develop Argyria using ONLY .999+ fine silver electrodes,(I do use .9999 Silver Maples in my CS) can you comprehend? Sorry I should not get petty but please read before you post.Sorry for being childish. distilled water, and low voltage. ( my voltage never exceeds around 14v DC however my amperage is very controlled though so I have a very repeatable process)Through stomach acid or sublingually, topical patch, mist inhaler or as a lavage.

    There is evidence where ONLY .999+ fine silver, distilled water, and low voltage( use that as well) HAS remedied mild cases of Argyria. Sorry but you can not reverse Argyria. "It is a photo chemical" (and I say that without full knowledge of the process) reaction I guess it could be bleached kinda like what Michael Jackson tried with his skin.
     
  36. abeland1

    abeland1 Silver Member Silver Miner

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    I'm going to print out the article from NIH:
    ABSTRACT
    The antibacterial effect and mechanism of action of a silver ion solution that was electrically generated were investigated for Staphylococcus aureus and Escherichia coli by analyzing the growth, morphology, and ultrastructure of the bacterial cells following treatment with the silver ion solution. Bacteria were exposed to the silver ion solution for various lengths of time, and the antibacterial effect of the solution was tested using the conventional plate count method and flow cytometric (FC) analysis. Reductions of more than 5 log10 CFU/ml of both S. aureus and E. coli bacteria were confirmed after 90 min of treatment with the silver ion solution. Significant reduction of S. aureus and E. coli cells was also observed by FC analysis; however, the reduction rate determined by FC analysis was less than that determined by the conventional plate count method. These differences may be attributed to the presence of bacteria in an active but nonculturable (ABNC) state after treatment with the silver ion solution. Transmission electron microscopy showed considerable changes in the bacterial cell membranes upon silver ion treatment, which might be the cause or consequence of cell death. In conclusion, the results of the present study suggest that silver ions may cause S. aureus and E. coli bacteria to reach an ABNC state and eventually die.

    Since ancient times, the silver ion has been known to be effective against a broad range of microorganisms. Today, silver ions are used to control bacterial growth in a variety of medical applications, including dental work, catheters, and the healing of burn wounds (17, 30, 31). Silver ions are also used for a number of nonmedical purposes, such as in electrical appliances (14, 36). The slow-release “nanosilver” linings of laundry machines, dishwashers, refrigerators, and toilet seats are also marketed and advertised. It is clear that we are exposed to a wide range of mostly unfamiliar uses of silver-containing products intended to function as antimicrobial biocides. Therefore, it is necessary to elucidate the antimicrobial activity of the silver ion, which is widely used in these products.

    The mechanism of the antimicrobial action of silver ions is closely related to their interaction with thiol (sulfhydryl) groups (1, 5, 9, 10), although other target sites remain a possibility (27, 34). Amino acids, such as cysteine, and other compounds containing thiol groups, such as sodium thioglycolate, neutralized the activity of silver against bacteria (18). By contrast, disulfide bond-containing amino acids, non-sulfur-containing amino acids, and sulfur-containing compounds, such as cystathione, cysteic acid, l-methionine, taurine, sodium bisulfate, and sodium thiosulfate, were all unable to neutralize the activity of silver ions. These and other findings imply that the interaction of silver ions with thiol groups in enzymes and proteins plays an essential role in its antimicrobial action, although other cellular components, like hydrogen bonding, may also be involved (10). Silver was also proposed to act by binding to key functional groups of enzymes. Silver ions cause the release of K+ ions from bacteria; thus, the bacterial plasma or cytoplasmic membrane, which is associated with many important enzymes, is an important target site for silver ions (9, 22, 25, 29).

    In addition to their effects on bacterial enzymes, silver ions caused marked inhibition of bacterial growth and were deposited in the vacuole and cell wall as granules (6). They inhibited cell division and damaged the cell envelope and contents of bacteria (27). Bacterial cells increased in size, and the cytoplasmic membrane, cytoplasmic contents, and outer cell layers all exhibited structural abnormalities. Finally, silver ions interact with nucleic acids (35); they interact preferentially with the bases in DNA rather than with the phosphate groups, although the significance of this in terms of their lethal action is unclear (12, 24, 34, 37).

    The following silver compounds and silver are listed in Martindale: the Extra Pharmacopoeia: silver metal, silver acetate, silver nitrate, silver protein, and silver sulfadiazine (26a). The silver ion can be generated by electrolyzing the silver metal or dissolving the silver compounds. It is known that the electrically generated silver ion appeared to be superior to the silver compounds in antimicrobial activity (3, 4). However, most of the aforementioned studies which determined a mechanism of action of silver used silver ions produced from silver compounds like silver nitrate or silver sulfadiazine, and thus there has been limited research on the electrically generated silver ion. Recently, a laundry machine that emits electrically generated silver ions was developed for hygiene, namely, in order to prevent easily transmissible bacterial and fungal skin infections from being transmitted by contaminated laundry. In particular, it can be beneficial to hospitals and homes in which immunocompromised people (the elderly, children, and medical patients) or pets may dwell. Our previous study demonstrated the antifungal activity of a laundry machine that electrically generates silver ions (14). In the present study, we used conventional plate counting, flow cytometry (FC), and transmission electron microscopy (TEM) to investigate the antibacterial activity and mechanism of action against Staphylococcus aureus and Escherichia coli bacteria of a silver ion solution generated from the laundry machine.

    Go to:
    MATERIALS AND METHODS
    Bacterial strains.
    S. aureus ATCC 25923 and E. coli ATCC 25922 were used in this study. The strains were grown in 5% sheep blood agar (Promed, Gyeonggi, Korea).

    Antibacterial efficacy test of household laundry machines.
    A silver laundry machine (Samsung, Gyeonggi, Korea) and a conventional laundry machine (Samsung) which was the same as the silver laundry machine except for the fact that it did not emit silver ions were used as the experimental and control machines, respectively. The silver laundry machine is designed to release silver ions twice during the laundry process: once during the main washing step (for 30 min) and once during the final rinsing step (for 20 min). Powerclean Max (Oxy, Seoul, Korea) was used as the detergent.

    The method for testing the antibacterial properties of household laundry machines was performed as previously described (17a) with minor revision. The bacteria were enumerated by the conventional plate count method. The test textile (100% cotton) was 5 cm × 5 cm. Three pieces of test textile were attached to the edge of a 1-m × 1-m laundry textile (100% cotton). Each test and laundry textile was autoclaved and dried, and then the test textiles were inoculated with S. aureus or E. coli. The bacteria were diluted to 109 to 1010 CFU/ml using 0.85% sterile saline. One milliliter of each adjusted bacterial culture was inoculated to the test textiles, and then textiles were washed in each laundry machine.

    Two pieces of laundry textile with three pieces of test textile and 28 pieces of laundry textile without test textile, which were used to adjust the weight of the laundry to be 3 kg, were processed at the same time with or without detergent using the silver and the conventional laundry machine. The laundry textile with the test textile attached to it was taken out at the end of the laundry process. The test textile was then removed from the laundry textile and pummeled with 10 ml of sterile buffered peptone water (Becton Dickinson, Sparks, MD). The buffered peptone water rinse solution was then serially diluted with saline, and bacteria were counted using the conventional plate count method.

    Silver ion preparation.
    A silver ion solution in phosphate-buffered saline (PBS; pH 7.4) was prepared from the silver laundry machine (Samsung), and this solution was used in all subsequent experiments (conventional plate counting, FC analysis, and TEM). The silver ions were produced from two silver plates while PBS was passed through the silver kit, which was made with polypropylene housing. The water from the tap passed through the silver kit housing and went down to the drum. Both the anode and cathode were 99.9% silver metal plates with surface areas of 12.5 cm2, and two electrodes were installed parallel to each other with 5 mm of distance between them. The volume of the silver kit housing was 30 ml. The flow rate through the silver kit housing was regulated to be 10 liter/min, and the electric current was controlled at 80 mA by changing the input voltages from 2 to 24 V. The electric current was applied only during water supply. The concentration of the silver was determined by inductively coupled plasma mass spectrometry (ELAN 6100; Perkin-Elmer SCIEX, Norwalk, CT) at the National Center for Inter-University Research Facilities, Seoul National University, and it was approximately 0.2 ppm.

    Determination of antibacterial effect of silver ions by conventional plate counting.
    The silver ion solution made with PBS was autoclaved at 121°C for 15 min and tested for its antibacterial efficacy. The concentrations of silver ions tested were 0.2, 0.1, and 0.05 ppm. Ninety-nine milliliters of the test solution and 1 ml of the bacterial suspension in PBS were mixed to a final bacterial concentration of 105 to 106 CFU/ml. The mixture of solution and bacteria was incubated at 37°C with shaking and counted at 30-min intervals from 30 to 180 min and then again at 24 h using the conventional plate count method, with serial 10-fold dilutions with saline plated on plate count agar (Becton Dickinson).

    FC analysis of antibacterial effect of silver ions.
    After the bacterial suspensions (105 to 106 CFU/ml) were treated with silver ion solution (0.2 ppm) or PBS for 30 min, 1 h, 1.5 h, 2 h, and 3 h, the bacterial cells (S. aureus or E. coli) were washed two times with PBS and resuspended in SYTO 9 and propidium iodide (PI) from a Live/Dead BacLight bacterial viability kit (Molecular Probes, Inc., Eugene, OR) (2, 28). The suspension was incubated for 15 min in the dark at room temperature. In the control group, suspensions of fresh live (untreated) and dead (70% isopropyl alcohol treated) cells were stained as described above, and the green and red fluorescence generated by SYTO 9 and PI staining, respectively, as well as the size (side scatter height) were also read by FC analysis. After reading the parameters of the live and dead cell controls, with the resulting live cells in gate 1 (R1-green) and damaged or dead cells in gate 2 (R2-red) as discriminated by FC analysis, the relative frequencies of cells in each gate before treatment with silver ion solution or PBS were determined, with all of the experimental profiles being analyzed with gates 1 and 2 by FC analysis. The green fluorescence of the SYTO 9 dyes (FL1) was collected using a 530-nm ± 30-nm band-pass filter. The red fluorescence emitted from PI (FL3) was collected using a 650-nm ± 13-nm band-pass filter. The proportions of live and dead cells were determined and analyzed by using a FACSCalibur with the CellQuest program (Becton Dickinson Immunocytometry Systems, San Jose, CA) and FCS Express software (De Novo Software, Ontario, CA), respectively.

    For the enumeration of esterase-active bacteria, 900 μl of bacterial cells, which were treated with silver ion solution or PBS and washed as described above, were supplemented with 90 μl of sterile 1.0 M phosphate buffer (pH 8.0) and 10 μl of 50 mM EDTA. Then, carboxyfluorescein diacetate (CFDA; Molecular Probes, Inc.) stock solution in dimethyl sulfoxide was added to the sample at a final concentration of 10 μM, and the sample incubated at 35°C in the dark for 10 min (11). Following incubation, the cells were washed and resuspended in sterile 1.0 M phosphate buffer (pH 8.0), and esterase-active bacteria were enumerated by the enhanced-green-fluorescence intensity as determined by FC analysis. Positive-control live cells and negative-control dead cells were prepared and stained as described above.

    TEM.
    Unstained cells of S. aureus and E. coli were observed for the presence of electron-dense precipitates by TEM. The two bacterial strains were diluted to a final concentration of 105 to 106 CFU/ml with silver ion solution (0.2 ppm) or PBS. The mixture of solution and bacteria was incubated at 37°C for 2 h with shaking, centrifuged at 1,320 × g for 30 min to obtain cell pellets, and then diluted with 1 ml of PBS. A drop of the mixture was placed on a glow-discharged Formvar-coated copper grid for 1 min. The excess liquid was drained off with a filter paper, and the preparation was air dried for 5 min. The specimens were examined with an energy-filtering TEM (LIBRA 120; Carl Zeiss, Oberkochen, Germany) operated at an accelerating voltage of 120 kV. Zero-loss energy-filtered images were recorded with a 4 K slow-scan charge-coupled-device camera (4000 SP; Gatan, Pleasanton, CA).

    In addition, the detailed ultrastructural changes induced by the silver ion treatment in embedded bacterial cells were examined. The cell pellets of the two bacterial strains were fixed with modified Karnovsky's fixative consisting of 2% (vol/vol) glutaraldehyde and 2% (vol/vol) paraformaldehyde in 0.05 M sodium cacodylate buffer (pH 7.2) at 4°C for 2 h (15). They were then washed three times with the same buffer for a period of 10 min. The specimens were postfixed with 1% (wt/vol) osmium tetroxide in the same buffer at 4°C for 2 h and washed briefly with distilled water twice. The postfixed specimens were dehydrated in a graded ethanol series (once in 30, 50, 70, 80, and 95% and three times in 100% for 10 min each). The specimens were further treated with propylene oxide twice each for 10 min as a transitional fluid and then embedded in Spurr's resin (33). Ultrathin sections (approximately 60-nm thickness) were cut with a diamond knife using an ultramicrotome (MT-X; RMC Inc., Tucson, AZ) and then mounted on bare copper grids. They were stained with 2% uranyl acetate and Reynolds' lead citrate (26) for 7 min each, followed by examination with the electron microscope.

    Statistical analysis.
    The data from triplicate experiments are presented as the mean ± standard error of the mean. An unpaired t test analysis was performed using Origin 6.1 (OriginLab, Northampton, MA) to compare the viable bacterial counts within different samples that underwent different washing treatments (detergent and laundry machines) and to compare the viable bacterial counts between the silver ion treatment and nontreatment groups. The proportions of live or dead E. coli or S. aureus determined by FC analysis in the silver ion treatment groups treated for different periods of time (30 min, 1 h, 1.5 h, 2 h, and 3 h) were compared with those in the control (PBS) group using the Kruskal-Wallis one-way analysis of variance by rank. Significant differences in the data that originated from the same group but were determined at different times were analyzed by the Wilcoxon signed-rank test using Analyze-it software (Analyze-it Software Ltd., Leeds, United Kingdom). The level of significance was set at a P value of <0.05.

    Go to:
    RESULTS
    Antibacterial efficacy of household laundry machines.
    The efficacy test results of the two laundry machines against S. aureus and E. coli conducted with or without detergent are shown in Fig. Fig.1.1. The S. aureus bacterial count was significantly reduced by the silver laundry machines with detergent in comparison to the results with the conventional laundry machine (P < 0.05). All of the inoculated E. coli bacteria were eliminated when detergent was used in both the silver and conventional laundry machines. In the absence of detergent, E. coli was significantly reduced by the silver laundry machine in comparison to the results with the conventional laundry machine (P < 0.05).

    FIG. 1.
    FIG. 1.
    Viable counts (mean ± standard error) of Staphylococcus aureus (a) and Escherichia coli (b) bacteria after washing bacteria-contaminated textile pieces using silver and conventional laundry machines with (W/) or without (W/O) detergent. Each group ...
    Effect of the silver ions on the bacterial reduction rate.
    The antibacterial effects of the silver ion solution at different concentrations of silver ions against S. aureus and E. coli bacteria as determined by the conventional plate count technique are shown in Fig. Fig.2.2. The total number of S. aureus bacteria was reduced by over 5 log10 CFU/ml after treatment with the original silver ion solution (0.2 ppm) for 90 min, demonstrating that the antibacterial activity of the silver ion solution was significantly greater than that of PBS treatment (P < 0.05). The E. coli bacterial count was reduced from the inoculum size (105 CFU/ml) to the limit of detection (<20 CFU/ml) within 30 min at a silver ion concentration of 0.2 ppm. All of the tested silver ion solutions (0.2, 0.1, and 0.05 ppm) significantly eliminated E. coli cells in comparison to PBS treatment (P < 0.05).

    FIG. 2.
    FIG. 2.
    The effect of the silver ion solution on Staphylococcus aureus (a) and Escherichia coli (b) was investigated by conventional plate counting. The tested silver ion concentrations were 0.2 ppm, 0.1 ppm, and 0.05 ppm, and PBS was used as a control.
    FC analysis in conjunction with a BacLight kit was also performed to examine the antibacterial effect of the original silver ion solution (0.2 ppm) against S. aureus and E. coli bacteria in terms of damage to the cell membrane, shown in different colors (green in live cells and red in damaged or dead cells). In addition, CFDA staining was used for the enumeration of esterase-active bacteria because CFDA is cell permeant and undergoes hydrolysis of the diacetate groups into fluorescent carboxyfluorescein by intracellular nonspecific esterases. Based on the side light scatter and green (FL1) fluorescence, the R1 and R2 gates were used to identify live and damaged or dead cells, respectively. The proportions of damaged or dead cells (both S. aureus and E. coli) in the silver ion solution-treated groups were significantly greater (P < 0.05) at 30 min, 1 h, 1.5 h, and 2 h of treatment than with the control (PBS) groups (Fig. (Fig.33 and and4).4). Longer treatment times (from 30 min to 2 h) had a positive effect on the antibacterial effect of the silver ion solution (P < 0.05); however, there were no significant differences in the proportions of live or dead cells when both S. aureus and E. coli cells were treated with the silver ion solution for 2 or 3 h (P > 0.05).

    FIG. 3.
    FIG. 3.
    Representative dot plot profiles of Staphylococcus aureus cells treated with PBS (e, g, i, and j) or silver ion solution (0.2 ppm) (Tx; f, h, k, and l) for 30 min, 1 h, and 2 h analyzed by FC after staining with SYTO 9 and PI. For controls, suspensions ...
    FIG. 4.
    FIG. 4.
    Representative dot plot profiles of Escherichia coli cells treated with PBS (c, e, and g) or silver ion solution (0.2 ppm) (Tx; d, f, and h) for 30 min, 1 h, and 2 h analyzed by FC after staining with SYTO 9 and PI. For controls, suspensions of fresh ...
    The antibacterial-efficacy results determined by conventional plate count and FC analyses are compared in Fig. Fig.5.5. For the PBS-treated control group and silver ion-treated experimental groups tested, both the BacLight kit and the CFDA assay gave similar antibacterial efficacies (P > 0.05). The number of physiologically active bacteria enumerated by FC analysis in conjunction with the BacLight kit or the CFDA assay was relatively higher than the bacterial count determined by conventional plate counting (P < 0.05), except for E. coli bacteria after 2 and 3 h of treatment. This difference appeared to be nonlinear across different treatment times, suggesting that the difference in antibacterial efficacy determined by the two analyses decreased as the silver ion treatment times approached 2 and 3 h.

    FIG. 5.
    FIG. 5.
    Comparative analysis of the antibacterial efficacy of the silver ion solution (0.2 ppm) against Staphylococcus aureus (a) and Escherichia coli (b) bacteria as determined using the conventional plate count method and FC analysis. PBS was used as a control. ...
    Morphological changes in S. aureus and E. coli cells after silver ion treatment.
    TEM analysis of unstained bacteria showed the external morphological features of the two bacterial strains. The untreated S. aureus cells retained their coccal morphology (ca. 600 nm in diameter) and seemed to be normal (Fig. (Fig.6a).6a). In contrast, S. aureus cells treated with the silver ion solution for 2 h appeared to undergo lysis, resulting in the release of their cellular contents into the surrounding environment, and finally became disrupted (Fig. 6b to d). It was common to find electron-dense particles or precipitates around damaged bacterial cells that were electron translucent in comparison to undamaged cells. In cross section, the untreated cells of S. aureus showed normal cell characteristics and homogeneous electron density in the cytoplasm. Their cell walls and membranes were intact, showing a well-preserved peptidoglycan layer and cytoplasmic membrane (Fig. 7a and b). However, significant morphological changes were observed in S. aureus cells treated with the silver ion solution. They showed lysed cells with broken walls and membranes and decreases and heterogeneity in electron density in the cytoplasm (Fig. 7c and d). The localized separation of the cell membrane from the cell wall could be discerned.

    FIG. 6.
    FIG. 6.
    External morphology of unstained Staphylococcus aureus cells observed by TEM. (a) Untreated bacteria. (b, c, and d) Bacteria treated with silver ion solution (0.2 ppm). Electron-dense particles were found around damaged cells (arrows). Note the release ...
    FIG. 7.
    FIG. 7.
    Internal structure of Staphylococcus aureus observed by TEM. (a and b) Untreated bacteria. (c and d) Bacteria treated with silver ion solution (0.2 ppm). Black and white arrows indicate peptidoglycan layer and cytoplasmic membrane, respectively. Note ...
    E. coli cells diluted in PBS showed normal morphology having many filaments, such as flagella and fimbriae (Fig. (Fig.8a).8a). The fimbriae were peritrichous, approximately 7 nm wide, and up to 900 nm long. Meanwhile, the bacterial cells after silver ion treatment for 2 h appeared to be seriously damaged (Fig. 8b to d). The cells showed aberrant morphology; they were cracked and ruptured. Electron-dense particles or precipitates were also observed around damaged bacterial cells. The internal structure of the untreated E. coli cells appeared to be normal, showing a multilayered cell surface consisting of an outer membrane, a peptidoglycan layer in the periplasmic space, and a cytoplasmic membrane (Fig. 9a and b). Damaged cells showed either localized or complete separation of the cell membrane from the cell wall (Fig. (Fig.9c).9c). The cellular degradation was also accompanied by electron-translucent cytoplasm and cellular disruption in the damaged cells (Fig. (Fig.9d9d).

    FIG. 8.
    FIG. 8.
    External morphology of unstained Escherichia coli observed by TEM. (a) Untreated bacteria. An arrow and an arrowhead indicate fimbriae and a flagellum, respectively. (b, c, and d) Bacteria treated with silver ion solution (0.2 ppm).
    FIG. 9.
    FIG. 9.
    Internal structure of Escherichia coli observed by TEM. (a and b) Untreated bacteria. (c and d) Bacteria treated with silver ion solution (0.2 ppm). Arrows indicate outer membrane, peptidoglycan layer, and cytoplasmic membrane from the outside of the ...
    Go to:
    DISCUSSION
    The electrically generated silver ion solution exhibited good bactericidal efficacy against S. aureus and E. coli both in experiments using the silver laundry machine with contaminated fabric and in those using the silver ion suspension generated from the silver laundry machine. The efficacy of the silver ion solution showed better activity against the gram-negative E. coli than against the gram-positive S. aureus. This was possibly due to the thickness of the peptidoglycan layer, which may prevent the action of the silver ions through the bacterial cell wall, and this result was consonant with the results of other studies (8, 23). Although the S. aureus and E. coli bacteria were effectively eliminated from the contaminated fabric by the silver washing course, it was not confirmed that the silver ions killed the bacteria. It is possible that the bacteria were removed from the fabric by the washing course. Therefore, the antibacterial effect of the silver ions was confirmed by the conventional plate count, FC, and TEM analyses in this study.

    The number of bacteria determined by conventional plate counting, which counts only culturable colonies in media, was significantly lower than the number determined by FC analysis, suggesting that the cell membrane and intracellular esterase activity of the bacteria treated with the silver ion solution might be damaged. Bacteria in the environment are exposed to various conditions that lead to survival stress. To counter this condition, some bacteria are capable of maintaining metabolic activity while developing recalcitrance to culture. Such a state in bacteria is often defined as an “active but nonculturable (ABNC)” state, a state in which the bacteria exhibit measurable traits of physiological activity but fail to grow to a detectable level (16). A state of ABNC or sublethal injury of bacteria seems to be induced by exposure to silver ions, thus rendering bacteria nonculturable in media (7, 21). This may serve as a possible explanation for the discrepancy in the results determined by the two methods used in this study, and this observation is consistent with the findings of other studies (11, 13). This finding may be expected because bacteria previously exposed to environmental stresses may only be able to divide a limited number of times, which would give a positive result in the FC analysis, but they would be unable to produce visible colonies on solid media.

    The differences between the results of the conventional plate count and FC analyses were nonlinear, and the difference rate between the results of the two methods was reduced as time progressed. The reason for this aspect might be that the bacteria in the ABNC state started to die after 2 h of treatment with the silver ions.

    Similar phenomena were also observed in the silver ion-treated cells of S. aureus and E. coli by the TEM studies. Following the silver ion treatment, the cytoplasm membrane shrank and became separated from the cell wall. Cellular contents were then released from the cell wall, and the cell wall was degraded. These phenomena suggest possible antibacterial mechanisms by which silver ions inhibit bacterial growth, as well as cellular responses of both the gram-positive and gram-negative bacteria to the silver ion treatment. Although the mechanisms underlying the antibacterial actions of silver are still not fully understood, several previous reports (20, 23, 32) showed that the interaction between silver and the constituents of the bacterial membrane caused structural changes and damage to the membranes and intracellular metabolic activity which might be the cause or consequence of cell death, as demonstrated in this study. Analytical electron microscopy remains to be done to identify the elemental composition of the electron-dense particles or precipitates around damaged bacterial cells. In conclusion, the results of the present study clearly show that the electrically generated silver ion solution exerts its antibacterial effect by inducing bacteria into a state of ABNC, in which the mechanisms required for the uptake and utilization of substrates leading to cell division were disrupted at the initial stage and caused the cells to undergo morphological changes and die at the later stage. These findings suggest that the use of the silver ion solution may have valuable applications in various fields, such as the manufacture of household appliances and medical devices.

    Go to:
    ACKNOWLEDGMENTS
    We thank Young Hwan Paik for his technical assistance.

    This study was supported by Korea Research Foundation grants (KRF-2006-005-J02903 and KRF-2007-331-E00254), a grant from the Technology Development Program for Agriculture and Forestry provided by the Ministry of Agriculture and Forestry (grant no. 305003-3), and the Korea Bio-Hub Program of the Korea Ministry of Commerce, Industry Energy (2005-B0000002). Additional support was provided by the Research Institute of Veterinary Science, Department of Veterinary Microbiology, College of Veterinary Medicine, and the BK21 Program for Veterinary Science, Seoul National University.
    Now, what does the foregoing say to you as to the efficacy of silver ions versus silver particles?
     
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  37. abeland1

    abeland1 Silver Member Silver Miner

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    This is from a very impressive New Zealand site:
    http://www.holistichealthglobal.co.nz/
    I thought I would let these people help explain the difference between ionic and particulate silver.

    "The Fine Line: Ionic vs Colloidal Silver
    When reading websites and some of the literature, colloidal silver and ionic silver are often used interchangeably, but there is a difference. Ions represent the active form of silver, whereas particulate (metallic) silver is inert. It may release ions under appropriate conditions, however.

    When atoms give off an electron (oxidation), they assume a less stable state. Silver exhibits three oxidation states, Ag+, Ag2+, and Ag3+, each subsequently losing another electron. Only the first, Ag+, is sufficiently stable for use as antibiotic, the others are too highly reactive, and thus short lived.

    Silver compounds ionize in water and biological tissues – they release Ag+ to some extent (Lansdown 2006). Thus, orally taken CS also delivers silver ions, together with nanoparticles (Munger et al. 2014a), and the mechanism of action likely always involves silver ions.

    Metallic silver (Ag) is inert and without biocidal activity. Your silverware or jewelry won’t externally affect your health – swallow that tongue piercing, however, and it becomes a source of silver ions as it passes through your digestive tract. Given the low silver ion concentrations known to affect microbiota – 1 part per million or less (Marx & Barillo 2014) – this actually could affect your intestinal flora. One would probably have to swallow lots of jewelry to feel any effect, however – so rest easy while awaiting its natural reappearance.

    Silver Biochemistry ~ How it Works
    To exhibit any antimicrobial effect, silver needs to occur in free, ionic form (Ag+). Bound to molecules (as complex chelates or precipitates), silver loses its biocidal capacity. Kind of like not being able to catch a volleyball when already holding one. You’re not free to accept another, and thus unavailable to interact any further. Ionic silver is highly reactive and readily combines with inorganic substrates (eg chloride), organic acids, negatively charged proteins, DNA, and RNA (Marx & Barillo 2014). Which brings us to how silver exerts its antimicrobial effect.

    In essence, silver (ions) bind to cell surface receptors of bacteria, yeasts, and fungi. While the exact mechanism of action remains unclear, four possible ways are suggested, and they all include microorganisms accumulating enough silver until a lethal concentration is reached: 1) Silver blocks and eventually shuts down vital enzyme systems; 2) Silver causes a cell wall or membrane to rupture or leak; this also potentially interferes with concentration gradients between the cell and its environment and may hinder nutrient uptake; 3) Silver directly causes DNA mutations in bacteria (their DNA is not protected by a surrounding nucleus, as in our cells); 4) Silver forms free radicals that cause havoc within the cell by damaging vital structures and molecules, eventually leading to its demise."

    This is only a part of what's on the webpage. A visit to this site could be a relaxing change in comparison to so many agenda driven sources of information on the subject of colloidal silver.
     
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  38. abeland1

    abeland1 Silver Member Silver Miner

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    The world is running out of antibiotics, WHO report confirms

    19 SEPTEMBER 2017 - The report – “Antibacterial agents in clinical development – an analysis of the antibacterial clinical development pipeline, including Mycobacterium tuberculosis” – shows a serious lack of new antibiotics under development to combat the growing threat of antimicrobial resistance. Most of the drugs currently in the clinical pipeline are modifications of existing classes of antibiotics and are only short-term solutions. The report found very few potential treatment options for those antibiotic-resistant infections identified by WHO as posing the greatest threat to health, including drug-resistant tuberculosis which kills around 250 000 people each year. In addition to multidrug-resistant tuberculosis, WHO has identified 12 classes of priority pathogens – some of them causing common infections such as pneumonia or urinary tract infections – that are increasingly resistant to existing antibiotics and urgently in need of new treatments.

    Antibacterial agents in clinical development - an analysis of the antibacterial clinical development pipeline, including tuberculosis.
    Read the report
    http://apps.who.int/iris/bitstream/10665/258965/1/WHO-EMP-IAU-2017.11-eng.pdf?ua=1
     
  39. Thecrensh

    Thecrensh Gold Member Gold Chaser

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    I have an oncologist colleague that I discussed CS with at some point last year. He said that CS is an anti-bacterial, but if ingested the enzymes in our stomach will neutralize the effects...at least internally. It's still effective as a topical agent...I've seen that first-hand. If he's right, then we're wasting it when we drink it.
     
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  40. GOLDBRIX

    GOLDBRIX God,Donald Trump,most in GIM2 I Trust. OTHERS-meh Site Supporter Platinum Bling

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    ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
    I think your "doc" friend is buying the "company line". EIS/CS WORKS on bouts of food poisoning ( personal testament on that) . That happens in the stomach. Any time I feel "queezey" after eating I start a protocol of an ounce every hour until the feeling has stopped.
    I do not have issues with the claim Good Bacteria in the gut being destroyed by EIS/CS. For me that is a non-issue.

    Plus if "doc" want to maintain his belief there is the IV route for serious amounts of infusion, the Cool Mist Vaporizers, hand held mist inhalers ( target advertised them last year). For us laymen inhalation into the lungs is the best and most effective way to get EIS/CS into the body.

    And I have mentioned this in several Alt. Med. threads . I use EIS in the water reservoir of my CPAP machine at night and that is a nightly use.

    Medical and Pharma have their agendas just like every organization. Med. Schools no longer educate on Silver and Big Parma fears losing their "cash cows".

    DYODD
     
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